RANKL (TNFSF11) Goat Polyclonal Antibody

CAT#: AP01114PU-S

RANKL (TNFSF11) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Sandwich: To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human sRANKL as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.
  Indirect: To detect hsRANKL by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.
  Western blot: To detect hsRANKL by Western blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: E. coli derived ( highly pure; > 98 %) recombinant human sRANKL
• Specificity: This antibody detects RANKL.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store the lyophilized antibody at -20 °C.
  Following reconstitution it is stable for two weeks at 2 - 8 °C.
  Frozen aliquots are stable for 6 months when stored at -20 °C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: Approx. 150 kDa
• Gene Name: tumor necrosis factor superfamily member 11
• Database Link:
  Entrez Gene 8600 Human
  UniProt ID O14788
• Background: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
• Synonyms: OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF