WNT3A Rabbit Polyclonal Antibody

CAT#: AP00591BT-N

WNT3A rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human WNT-3a by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
  Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (Cat.-No AP00591PU-N or AP00591PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
  Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.Coli derived recombinant Human WNT-3a
• Specificity: This antibody detects Human WNT-3a. Other species not tested.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Wnt family member 3A
• Database Link:
  Entrez Gene 89780 Human
  UniProt ID P56704
• Synonyms: Protein Wnt-3a
• Note: Centrifuge vial prior to opening.