SIGLEC5 / SIGLEC14 Mouse Monoclonal Antibody [Clone ID: 1A5]

CAT#: AM60041PU-S

SIGLEC5 / SIGLEC14 mouse monoclonal antibody, clone 1A5, Purified

Product Images

Human peripheral blood monocytes stained with SIGLEC5 / SIGLEC14 antibody Cat.-No. AM60041PU-S followed by secondary FITC conjugated antibody.

Specifications

Product Data

• Clone Name: 1A5
• Applications: ELISA, FC, IF, IP
• Recommended Dilution: Flow cytometry: 1/25-1/200, use 10µl of this working dilution to label 10e6 cells in 100µl.
  Immunofluorescence.
  Immunoprecipitation.
  ELISA.
• Reactivities: Chimpanzee, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1
• Specificity: This antibody recognises human SIGLEC5 / CD170. Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).
• Formulation: PBS containing 0.09% Sodium azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.
  Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases.
  Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.
• Synonyms: Q08ET2, O15389, CD170