GNMT Mouse Monoclonal Antibody [Clone ID: AT5D1]

CAT#: AM50630PU-N

GNMT mouse monoclonal antibody, clone AT5D1, Purified

Product Images

The Recombinant protein (50ng) and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. GNMT recombinant protein. Lane 2: Liver tissue lysate. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate, Lane 2: GNMT Transfected 293T cell lysate.

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  ICC/IF analysis of GNMT in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT5D1
• Applications: ELISA, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human GNMT (1-295aa) purified from E. coli.
• Formulation: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: glycine N-methyltransferase
• Database Link:
  Entrez Gene 27232 Human
  UniProt ID Q14749
• Background: Ras-related protein Glycine N-methyltransferase (GNMT) catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
• Synonyms: Glycine N-methyltransferase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycine, serine and threonine metabolism