14 3 3 gamma (YWHAG) Mouse Monoclonal Antibody [Clone ID: AT4B9]

CAT#: AM50336PU-N

14 3 3 gamma (YWHAG) mouse monoclonal antibody, clone AT4B9, Purified

Specifications

Product Data

• Clone Name: AT4B9
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  WB: 1/1000 (using lysates of HeLa (40µg) and mouse brain (40 µg), resolved by SDS-PAGE and transferred to PVDF membrane)
  ICC/IF: 1/100 (using HeLa cell line).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli.
• Specificity: Recognizes Human 14-3-3 gamma (YWHAG). Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
• Database Link:
  Entrez Gene 7532 Human
  UniProt ID P61981
• Background: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
• Synonyms: YWHAG, KCIP-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis