TFF1 Mouse Monoclonal Antibody [Clone ID: SPM573]

CAT#: AM50172PU-T

TFF1 mouse monoclonal antibody, clone SPM573, Purified

Product Images

Formalin-paraffin human breast Ca Stained with pS2 Monoclonal Antibody (Clone SPM573).

Specifications

Product Data

• Clone Name: SPM573
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen and/or progesterone receptor.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide of 28 amino acid residues corresponding to amino acids 57-84 from the C-terminus of Human pS2.
• Specificity: It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of Human pS2 protein.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 6.5 kDa
• Gene Name: trefoil factor 1
• Database Link:
  Entrez Gene 7031 Human
  UniProt ID P04155
• Background: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2.
  Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
• Synonyms: TFF1, BCEI, Breast cancer estrogen-inducible protein, PNR-2