LOC105369261 Mouse Monoclonal Antibody [Clone ID: SPM503]

CAT#: AM50171PU-S

LOC105369261 mouse monoclonal antibody, clone SPM503, Purified

Product Images

Formalin-paraffin spleen stained with CD43 Antibody (Clone SPM503).

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  CD43 expression by lymphocyte gated population of PBMC: PBMC stained either with CD43 Antibody (Clone SPM503) or isotype control.

Specifications

Product Data

• Clone Name: SPM503
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coaating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 1-2 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Paracortex in a tonsil or a reactive lymph node.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Myeloblastic KG1 cells.
• Specificity: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43.
  No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 95, 115, or 135 kDa
• Database Link:
  Entrez Gene 105369261 Human
  UniProt ID P16150
• Synonyms: Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN