Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: SPM286]

CAT#: AM50169PU-S

Melanoma gp100 (PMEL) mouse monoclonal antibody, clone SPM286, Purified

Product Images

Formalin-Paraffin Human melanoma tissue stained with gp100 Antibody (Clone SPM286).

Specifications

Product Data

• Clone Name: SPM286
• Applications: FC, IF, IHC
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Immunohistochemistry on Frozen and Formalin-fixed Sections: 1-2 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: SK-MEL-28 cells or Melanoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Membranes from a human melanoma metastasis.
• Specificity: By Immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 90-100 kDa
• Gene Name: premelanosome protein
• Database Link:
  Entrez Gene 6490 Human
  UniProt ID P40967
• Synonyms: SILV, D12S53E, PMEL17, ME20-M