Fibronectin (FN1) Mouse Monoclonal Antibody [Clone ID: SPM539]

CAT#: AM50111PU-T

Fibronectin (FN1) mouse monoclonal antibody, clone SPM539, Purified

Product Images

Formalin-fixed, paraffin-embedded Human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (Clone SPM539).

Specifications

Product Data

• Clone Name: SPM539
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: SW156 cells or Kidney.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose.
• Specificity: This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
  Cellular Localization: Connective tissue matrix.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 220 kDa (monomer); 440 kDa (dimer)
• Gene Name: fibronectin 1
• Database Link:
  Entrez Gene 2335 Human
  UniProt ID P02751
• Background: Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren’s disease.
  Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing.
• Synonyms: FN1, Cold-insoluble globulin, CIG