DNASE1 Mouse Monoclonal Antibody [Clone ID: 6D5]

CAT#: AM33438PU-N

DNASE1 mouse monoclonal antibody, clone 6D5, Aff - Purified

Product Images

Western Blot testing of anti-bovine DNaseI monoclonal antibody (6D5). Line 1: PageRuler Prestained Protein Ladder (#[SM0671] Fermentas); Line 2: Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of cell culture supernatant was loaded into the gel.

Specifications

Product Data

• Clone Name: 6D5
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/125 000 for Bovine DNase I.
  Western Blot: 1/5000-1/10000 for detection of Bovine DNase I, 1/1000 for Human DNase I.
• Reactivities: Bovine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full length Bovine DNaseI produced using CHO-based.
  Antigen is purified from cell culture supernatant.
• Specificity: Recognizes Bovine DNase I.
  Cross reactsvity to Human.
• Formulation: PBS pH 7.4
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 282217 Bovine
  UniProt ID P00639
• Background: Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.
• Synonyms: DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I