Fibronectin (FN1) Mouse Monoclonal Antibody [Clone ID: HFN7.1]

CAT#: AM33330PU-T

Fibronectin (FN1) mouse monoclonal antibody, clone HFN7.1, Purified

Specifications

Product Data

• Clone Name: HFN7.1
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Biological Blockade: Use Azide Free Antibody.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: SW156 cells or Kidney.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Fibronectin purified from serum by Affinity Chromatography on gelatin-sepharose.
• Specificity: This antibody recognizes Human Fibronectin. Other species not tested.
  Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing.
  This Monoclonal Antibody is directed against the peptide core and reacts with both the plasma and cellular forms of Fibronectin. It blocks the Fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of Fibronectin to its receptor on the cell surface.
  Cellular Localization: Connective tissue matrix.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 220 kDa (monomer); 440 kDa (dimer)
• Gene Name: fibronectin 1
• Database Link:
  Entrez Gene 2335 Human
  UniProt ID P02751
• Background: Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They occur as dimers of two 250 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
• Synonyms: FN1, Cold-insoluble globulin, CIG