beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 4C2]

CAT#: AM33002PU-N

beta Actin (ACTB) mouse monoclonal antibody, clone 4C2, Purified

Product Images

Methanol fixed human dermal fibroblast immunostained with 4C2 (1/500)

• 

  Immunofluorescence staining of 1 month old zebrafish embryo.

• 

  Immunofluorescence staining of 3 days old zebrafish embryo

Specifications

Product Data

• Clone Name: 4C2
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Flow cytometry.
  Western blot.
  Immunocytochemistry.
  Immunohistochemistry on Frozen sections.
  Immunohistochemistry on Paraffin-embedded sections.
  Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.
• Reactivities: Chicken, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide comprising the N-terminal nonapeptide of β-Cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue
• Specificity: This 4C2 Monoclonal antibody is highly specific for β-Cytoplasmic Actin.
  The antibody does not cross react with other Actin isoforms.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: actin, beta
• Database Link:
  Entrez Gene 60 Human
  UniProt ID P60709
• Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates.
  β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.
• Synonyms: Actin cytoplasmic 1, Beta-Actin