CD55 Mouse Monoclonal Antibody [Clone ID: PD2]

CAT#: AM32979PU-N

CD55 mouse monoclonal antibody, clone PD2, Purified

Specifications

Product Data

• Clone Name: PD2
• Applications: ELISA, FC, WB
• Recommended Dilution: Immuno Assays (Ref.1).
  Flow Cytometry (Ref.2).
  Western blot (Ref.1).
  Positive Control: Porcine platelet lysate.
  Typical starting working dilution: 1/50.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Pig DAF-Ig fusion protein.
• Specificity: The monoclonal antibody PD2 recognizes Pig complement decay accelerating factor (DAF), also designated as CD55.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction with 0.02% Sodium Azide
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: CD55 molecule (Cromer blood group)
• Database Link:
  UniProt ID P08174
• Background: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Pig DAF (CD55) is a 45-52 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells.
  Together with other complement regulators DAF protects self cells from autologous complementmediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.
• Synonyms: CR; CROM; DAF; TC