Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: NKI-SAM1]

CAT#: AM32393PU-N

Integrin alpha 5 (ITGA5) mouse monoclonal antibody, clone NKI-SAM1, Purified

Product Images

Flow Cytometric analysis of human monocytes showing CD49e positive population

Specifications

Product Data

• Clone Name: NKI-SAM1
• Applications: FC, IHC
• Recommended Dilution: Fluorescence experiments: NKI-SAM-1 is suitable for Flow Cytometry (using 0.5-1 µg for 10⁶ cells) and can be used to determine the VLA-5 expression in peripheral blood monocytes and monocyte derived dendritic cells B cells and B-cell precursors.
  Immunohistochemistry on Frozen Sections: 1/20 (Acetone Fixation).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody recognizes a 150/130 kD receptor for fibronectin, (VLA-alpha-5 protein from the integrin superfamily of antigens).
  It reacts with platelets, lymphocytes, monocytes and most cell lines including K562 and U937.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 5
• Database Link:
  Entrez Gene 3678 Human
  UniProt ID P08648
• Background: Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their environment, being both other cells or the extra- cellular matrix. Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated subunits, i.e. an α-subunit of 95 kDa that is conserved through the superfamily and a more variable β subunit of 150-170 kDa. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. The integrin α5 chain (160 kDa) (CD49e), undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light (25 kDa) and heavy (135 kDa) chains, which non-covalently associate with the integrin β1 subunit (CD29) (130 kDa), thus forming the heterodimer α5-β1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor (FNr) that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5 mediates binding of T- and B-cells to fibronectin, an extracellular matrix glycoprotein involved in cell adhesion and migration in wound healing, embryonic development and malignant transformation. The VLA-5 or FNr binds only fibronectin recognizing the Arg-Gly-Asp (RGD) sequence in the central region of the molecule.
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA