TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 52B83]

CAT#: AM32222PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone 52B83, Purified

Specifications

Product Data

• Clone Name: 52B83
• Applications: ELISA, FN, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Flow Cytometry.
  Immunohistochemistry on frozen and paraffin sections.
  The typical starting working dilution is 1:50.
  Immunoprecipitation.
  Immunoassay.
• Reactivities: Guinea Pig, Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody 52B83 reacts with Tumor Necrosis Factor alpha (TNF-alpha).
  The antibody cross reacts with mouse, guinea pig and rhesus monkey TNF-alpha.
  
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction (0.2 um filtered)
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Background:

  Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of microorganisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-receptors leading to receptor crosslinking.

• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin