TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: T1]

CAT#: AM32017BT-N

TNF alpha (TNF) mouse monoclonal antibody, clone T1, Biotin, Purified

Specifications

Product Data

• Clone Name: T1
• Applications: FN, IP, WB
• Recommended Dilution: Western blotting: Use 1/10 as a starting dilution.
  Flow Cytometry: Use 1/10 as a starting dilution.
  Immuno Assays.
  Immunoprecipitation.
  Inhibition of the Biological Activity: For Neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) Human TNF-alpha.
  The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-apha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody Cat.-No AM32018 clone T3, which recognizes soluble, membrane and receptor bound TNF-alpha
• Formulation: PBS
  Label: Biotin
  State: Purified
  State: Liquid (0.2 μm filtered) Ig fraction Pres. State:
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Background: Tumor necrosis factor (TNF, cachexin or cachectin and formally known as tumor necrosis factor alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis, and viral replication. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases, as well as cancer.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin