EBFP Mouse Monoclonal Antibody [Clone ID: 3A6]

CAT#: AM32008PU-N

EBFP mouse monoclonal antibody, clone 3A6, Purified

Product Images

Specificity of Anti-Fluorescent Protein Immunoglobulin, clone 3A6, determined by immunoblot analysis. Blot contains total cell extract containing recombinant EGFP (predicted band size 27 kDa). Incubated with antibody fraction (0.5 mg/ml) 1000X diluted in P

Specifications

Product Data

• Clone Name: 3A6
• Applications: ELISA, WB
• Recommended Dilution:

  ELISA.
  Immunoblotting.

• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
• Specificity:

  Specificity has been tested in ELISA and immunoblotting.
  In immunoblot the antibody cross reacts with YFP.
  Additional tests for cross reactivity have not yet been performed.

• Formulation: 10 mM Ammonium Bicarbonate buffer
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 2 mg BSA
• Purification: Protein A Chromatohgraphy
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
  
• Stability: Shelf life: One year from despatch.
• Background: Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.
• Synonyms: Enhanced Blue Fluorescent Protein