Cd27 Hamster Monoclonal Antibody [Clone ID: LG.3A10]

CAT#: AM31861BT-N

Cd27 hamster monoclonal antibody, clone LG.3A10, Biotin

Specifications

Product Data

• Clone Name: LG.3A10
• Applications: FC, FN, IHC
• Recommended Dilution: Flow Cytometry.
  Functional studies (stimulating).
  Immunohistochemistry on Frozen Sections.
  
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: BALB/c
  Cell Concentration : 1x10⁶ cells per tests
  Antibody Concentration Used: 0.5 μg/10⁶ cells
  Isotypic Control: PE Hamster IgG
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: MCD27 tag expressing clones AR0524 and AR0530
  Donor: Armenian hamster spleen
  Fusion Partner: SP2/0 Murine Myeloma Partner
• Specificity: This Monoclonal Antibody is specific for the Mouse CD27 receptor.
• Formulation: PBS
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD27 antigen
• Database Link:
  Entrez Gene 21940 Mouse
  UniProt ID P41272
• Background: CD27 is part of the Tumor Necrosis Factor (TNF) family of receptors and is lymphocyte specific. Murine CD27 is expressed on the great majority of both αβ and γδ T lymphocytes, on a small population of peripheral B cells and on a very small subset of B220+ cells in the bone marrow. Murine CD27 is found on all thymocytes except a subset of CD4-CD8-precursors. There are two known basic functions of CD27 signaling. First, the binding of CD27 to CD70, a protein which is also expressed in T and B cells, provides costimulatory signals in lymphocyte proliferation and immunoglobulin production. Second, the association of CD27 with intracellular Siva, a proapoptotic protein, results in the induction of apoptotic events.
• Synonyms: TNFRSF7, CD27L receptor, T14