UVRAG (389-699) Mouse Monoclonal Antibody [Clone ID: 1H4]

CAT#: AM26654AF-N

UVRAG (389-699) mouse monoclonal antibody, clone 1H4, Azide Free

Product Images

Western blot analysis of UVRAG expression in HeLa (1), Jurkat (2), NIH/3T3 (3), WR19L (4), Rat1 (5) and CHO (6) using AM26654AF-N.

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  Immunoprecipitation of UVRAG from HeLa (1, 2) and NIH/3T3 (3, 4) with mouse IgG1 (1, 3) or AM26654AF-N (2, 4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblottedwith AM26654AF-N.

Specifications

Product Data

• Clone Name: 1H4
• Applications: IP, WB
• Recommended Dilution: Western blot: 1 μg/ml for chemiluminescence detection system.
  Positive Control: HeLa, Jurkat (Human), NIH/3T3, WR19L (Mouse), Rat1 (Rat), and CHO (Hamster).
  Immunoprecipitation: 5 μg/300 μl of cell extract from 1 x 10⁷ cells.
• Reactivities: Hamster, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein corresponding to amino acid residues 389 to 699 of Human UVRAG
• Specificity: This antibody reacts with UVRAG.
• Formulation: PBS containing 50% glycerol, pH 7.2. No preservative is contained.
  State: Azide Free
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Agarose
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliqouts) at -20°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: UV radiation resistance associated
• Database Link:
  Entrez Gene 7405 Human
  UniProt ID Q9P2Y5
• Background: Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. UVRAG (UV irradiation resistance-associated gene) has been identified as an essential component of the Beclin 1-PI3KC3 complex that suppresses tumorigenicity of human colon and promotes Beclin 1 dependent autophagy.
• Synonyms: p63
• Note:

  This product was originally produced by MBL International.