TLR4 Mouse Monoclonal Antibody [Clone ID: 3C3]

CAT#: AM26183FC-N

TLR4 mouse monoclonal antibody, clone 3C3, FITC

Specifications

Product Data

• Clone Name: 3C3
• Applications: FC
• Recommended Dilution: Flow cytometry (Typical starting working dilution is 1:50).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CHO cells expressing chimeric human TLR4/MD-2 fusion protein
• Specificity:
  This antibody 3C3 reacts with human Toll-like receptor 4 (TLR4, CD284).
  
  It is TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.
• Formulation: PBS
  Label: FITC
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein G
• Conjugation: FITC
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: toll like receptor 4
• Database Link:
  Entrez Gene 7099 Human
  UniProt ID O00206
• Background:

  TLRs belong to a family of proteins that specifically recognize and sense microbial products. They are highly conserved throughout evolution. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. TLRs identified as type I transmembrane signalling receptors act as innate immune recognition receptors against many pathogens.TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria.

• Synonyms: Toll-like receptor 4

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway