EEF1A1 (N-term) Mouse Monoclonal Antibody [Clone ID: 900]
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Specifications
Product Data | |
Clone Name | 900 |
Applications | ELISA, IF, WB |
Recommended Dilution | Western blot: 1:50 as starting dilution. Immunoflourescence. Immuno assays. |
Reactivities | Bacteria |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | E. coli K-12 C600 Rif ( pKT146) bacteria |
Specificity | The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE). |
Formulation | PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide |
Concentration | lot specific |
Purification | Protein G purified |
Conjugation | Unconjugated |
Storage | Product should be stored at 2-8°C. |
Stability | Shelf life: one year from despatch. |
Gene Name | eukaryotic translation elongation factor 1 alpha 1 |
Database Link | |
Background | EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. |
Synonyms | EF-1-alpha-1, eEF1A-1, EF-Tu, EEF1A, EF1A, CCS3, PTI1, CCS-3, FLJ25721, GRAF-1EF, Elongation factor 1 alpha 1 |
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