HPV11 E2 protein (202-284) Mouse Monoclonal Antibody [Clone ID: 10E12]

CAT#: AM26113PU-N

HPV11 E2 protein (202-284) mouse monoclonal antibody, clone 10E12, Aff - Purified

Product Images

Western blot analysis of HPV-11 E2 protein. WB was carried out with 10E12 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11 E2 protein, lines 2 and 3 Nontransfected U2OS and Hela cell

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  IF analysis of HPV-11 E2 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleuslocalized HPV-11 E2 detected by Mab 10E12. IF analy

Specifications

Product Data

• Clone Name: 10E12
• Applications: ELISA, IF, WB
• Recommended Dilution: Immunoflourescence.
  Titer using recombinant HPV-11 E2- protein:
  ELISA: 1:32 000.
  Western blot: 1:1000.
  The antibody does NOT work in Immunoprecipitation!
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant full-length HPV-11 E2-protein purified from E. coli
• Specificity: This antibody detects HPV-11 E2 protein at aa 202-284.
  Reacts in WB and ELISA with HPV-11 native E2-protein as well recombinant E2-protein expressed in bacteria and Cos-7 cells.
• Formulation: PBS pH 7.4, with 0.09% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P04015
• Background: Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
• Synonyms: Human papilloma virus type 11, HPV-11 E2, HPV11 E2