HPV18 E2 protein (1-83) Mouse Monoclonal Antibody [Clone ID: 2E7]

CAT#: AM26111PU-N

HPV18 E2 protein (1-83) mouse monoclonal antibody, clone 2E7, Aff - Purified

Product Images

Western-Blot analysis of HPV-18 E2protein. WB was was carried out with 2E7 Mab using thelysates of U20S and Hela cells. Lanes 1 and 4 areaccording cells transfected with plasmid expressing HPV-11 E2 protein, lanes 2 and 3 are plain U2OS and Helacells. Last

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  Immunofluorescence analysis of HPV-18 E2 protein in U2OS cells. IF was carried out with Hela cells transfected with plasmid expressing HPV-18 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV-18 E2 detected by Ma

Specifications

Product Data

• Clone Name: 2E7
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA, Western Blot, Immunofluorescence.
  Titer using recombinant HPV-11 E2- protein:
  ELISA: 1/2000.
  Western Blot: 1/1000.
  The antibody does Not work in Immunoprecipitation!
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant full-length HPV-18 E2-protein purified from E. coli.
• Specificity: This antibody detects HPV-18 E2 protein at 1-83.
  Reacts in Western Blot and ELISA with HPV-18 native E2-protein as well recombinant E2-protein expressed in bacteria and Cos-7 cells.
• Formulation: PBS pH 7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P06790
• Background: Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
• Synonyms: Human papilloma virus type 18, HPV-18 E2, HPV18 E2