Prion protein PrP (PRNP) Mouse Monoclonal Antibody [Clone ID: EM-20]

CAT#: AM26026PU-N

Prion protein PrP (PRNP) mouse monoclonal antibody, clone EM-20, Aff - Purified

Product Images

Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment

Specifications

Product Data

• Clone Name: EM-20
• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.5 μg/ml (Non-reducing conditions are essential).
  This clone has been described to work in Immunohistochemistry on Paraffin Sections (10 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human prion protein
• Specificity: The Mouse monoclonal antibody EM-20 recognizes Human prion protein (PrP). Diglycosylated form of PrP has ~40 kDa, monoglycosylated form ~30 kDa, and nonglycosylated form ~19-21 kDa.
  This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: prion protein
• Database Link:
  Entrez Gene 5621 Human
  UniProt ID F7VJQ1
• Background: The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
• Synonyms: Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Prion diseases