CD19 Mouse Monoclonal Antibody [Clone ID: 4G7]

CAT#: AM26006RD5-N

CD19 mouse monoclonal antibody, clone 4G7, PE-DL594

Specifications

Product Data

• Clone Name: 4G7
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (0.4 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CCL (chronic lymphocytic leukemia) cells
• Specificity: This antibody recognizes CD19 (B4), a 95 kDa type I transmembrane glycoprotein of immunoglobulin superfamily, expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE-DL594
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
  Label: Conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE-DL594
• Storage: Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD19 molecule
• Database Link:
  Entrez Gene 930 Human
  UniProt ID P15391
• Background: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
• Synonyms: Leu-12, B-cell marker
• Note: DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.