RFP-Tag Mouse Monoclonal Antibody [Clone ID: RFP. H8]

CAT#: AM20837PU-N

RFP-Tag mouse monoclonal antibody, clone RFP. H8, Aff - Purified

Product Images

RFP Monoclonal Antibody dilution at 1/100 (1 g/ml) probed against HEK293 cells transfected with RFP-tagged protein vector. Lane 1: Untransfected Control. Lane 2: Transfected with Turbo-RFP. Lane 3: Transfected with DeRed

Specifications

Product Data

• Clone Name: RFP. H8
• Applications: IP, WB
• Recommended Dilution:
  Immunoprecipitation.
  Dot Blot.
  Western Blot: Use at 1/1,000 for 1 hour at room temperature using ECL development.
  Sensitivity: 1-10 ng of purified RFP or RFP fusion proteins (Western blot developed with ECL).
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: RFP from Discosoma and anemone N-terminal peptide-KLH conjugates.
• Specificity: This antibody recognizes native and denatured forms of RFP and its variants tag-RFP, turbo-RFP, DeRed, mCherry and mOrange.
• Formulation: 10mM PBS, PH 7.2 with 0.09% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q9U6Y8
• Background: Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc) which become attractive tools for a number of applications. For example the combination of green and red fusion proteins allows multicolor tracking as well as protein-protein interaction studies in living cells.
• Synonyms: Red fluorescent protein Tag, DsRed Tag