p53 (TP53) Mouse Monoclonal Antibody [Clone ID: IMD-53]

CAT#: AM20653PU-N

p53 (TP53) mouse monoclonal antibody, clone IMD-53, Purified

Product Images

Western Blot: HL-60 cell lysis

• 

  Immunohistochemistry on Human mammary cancer sections

Specifications

Product Data

• Clone Name: IMD-53
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 0.5-1 μg/ml.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections: Use at 0.5-2 μg/ml to detect p53 in formalin or acetone fixed tissues.
  Immunohistochemistry on Paraffin Sections: Use at 0.5-2 μg/ml to detect p53 in formalin fixed and paraffin embedded tissues.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human wild-type p53 protein.
• Specificity: This antibody reacts to p53 (TP53).
• Formulation: 1.2% Sodium Acetate with 2 mg BSA and 0.01 mg Sodium Azide as preservative
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% Sodium Acetate or neutral PBS
• Concentration: 0.1 mg/ml (after reconstitution with PBS)
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway