TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: Mab1]

CAT#: AM20447PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone Mab1, Purified

Specifications

Product Data

• Clone Name: Mab1
• Applications: ELISA, FN, WB
• Recommended Dilution:
  Western blot.
  ELISA: Can be used as capture antibody in combination with biotinylated antibody MAb11.
  Functional application: Neutralization.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human TNF-alpha
• Specificity: This antibody recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid Ig fraction
  Preservative: 15mM Sodium azide
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; > 95% pure by SDS-PAGE
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Background: TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus