CD11a / ITGAL Rat Monoclonal Antibody [Clone ID: M17/4]

CAT#: AM12064PU-N

CD11a / ITGAL rat monoclonal antibody, clone M17/4, Aff - Purified

Product Images

Surface staining of murine splenocytes using anti-CD11a monoclonal antibody (cloneM17/4) purified.

Specifications

Product Data

• Clone Name: M17/4
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: 1 µg/ml.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections (Acetone fixation).
  Positive Control: Mouse spleen or thymus.
  Functional Assays: Blocking.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
• Specificity: The antibody reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P24063
• Background: CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
• Synonyms: Integrin alpha-L, LFA1, LFA-1