beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 4E8H3]

CAT#: AM11016PU-N

beta Actin (ACTB) mouse monoclonal antibody, clone 4E8H3, Purified

Product Images

Figure 1. Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL60 and Jurkat cell line lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.

Specifications

Product Data

• Clone Name: 4E8H3
• Applications: WB
• Recommended Dilution: ELISA.
  Western blotting: 1/2000-1/10000.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: ACTB recombinant protein is used to produce this monoclonal antibody.
• Specificity: This antibody is specific to Beta-Actin.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: actin, beta
• Database Link:
  Entrez Gene 60 Human
  UniProt ID P60709
• Background: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Beta-actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
• Synonyms: Actin cytoplasmic 1, Beta-Actin