HA Epitope Tag (YPYDVPDYA) Mouse Monoclonal Antibody [Clone ID: 12CA5]

CAT#: AM11008SU-N

HA Epitope Tag (YPYDVPDYA) mouse monoclonal antibody, clone 12CA5, Ascites

Product Images

Figure 1. HA-tagged recombinant protein was expressed in COS7 cells. 15 g of cell lysate was loaded onto a gel to test the HA tag antibody (AM11008SU-N).

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  Figure 2. Western blot analysis of anti-HA Tag Antibody in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.

Specifications

Product Data

• Clone Name: 12CA5
• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  ChIP (See Ref.1).
  Immunoprecipitation (See Ref.2).
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
• Specificity: This monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
• Formulation: State: Ascites
  State: Liquid
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein G column, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Epitope tags consisting of short sequences recognized by well-characterizated Monoclonal Antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples.