beta III Tubulin (TUBB3) (tubulin beta-3 chain) Mouse Monoclonal Antibody [Clone ID: 3D9]

CAT#: AM10229PU-N

beta III Tubulin (TUBB3) (tubulin beta-3 chain) mouse monoclonal antibody, clone 3D9, Purified

Product Images

Western Blot using Tubulin beta-3 antibody : Lane 1: 100 ug MDA cell extract. Lane 2: 50 ug MDA cell extract.

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  Breast cancer stained in Immunohistochemistry with Tubulin beta-3 antibody

Specifications

Product Data

• Clone Name: 3D9
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution:

  ELISA.
  Western Blot: 1/500-1/1000.
  Immunoprecipitation: 1/50-1/200.
  Immunohistochemistry: 1/100-1/500.

• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: beta-Tubulin protein.
• Specificity: Reacts with Human 55 kDa TUBB3 protein.
  Cross reacts with Mouse, Rat and other species.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified antibody
  Preservative: None
• Reconstitution Method: Restore in distilled water
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tubulin beta 3 class III
• Database Link:
  Entrez Gene 10381 Human
  UniProt ID Q13509
• Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, α- and β-tubulin, are usually products of separate genes. The β-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III β-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III β-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4