Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: 11C2]

CAT#: AM10208SU-N

Nitrotyrosine mouse monoclonal antibody, clone 11C2, Supernatant

Specifications

Product Data

• Clone Name: 11C2
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution:

  ELISA: 1/100-1/1000.
  Western Blot: 1/100-1/500.
  Immunocytochemistry: 1/100-1/500.
  Immunohistochemistry: 1/100-1/500.

• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: KLH-Nitrotyrosine
• Specificity: Reacts with free and bound Nitrotyrosine.
  No cross reaction with free or bound Tyrosine
• Formulation: State: Supernatant
  State: Lyophilized Cell Culture Supernatant
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.
• Synonyms: NO-Tyrosine, Nitro-Tyrosine