IL32 (1-131) Mouse Monoclonal Antibody [Clone ID: AT2F9]

CAT#: AM09356PU-N

IL32 (1-131) mouse monoclonal antibody, clone AT2F9, Purified

Product Images

Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of Jurkat (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT2F9
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:1000).
  Immnocytochemistry / Immunoflourescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human IL32 (1-131aa) purified from E. coli
• Specificity: The antibody recognizes human IL-32 at aa1-131. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 32
• Database Link:
  Entrez Gene 9235 Human
  UniProt ID P24001
• Background: The gene encoding IL32 is located on human chromosome 16p13.3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNFα and IL1β in murine peritoneal macrophages as well as in phorbol ester-differentiated human THP1 cells. In the inflamed lesions of chronic pancreas, the ductal expression of IL32 was markedly increased. Pancreatic duct cells are the local source of IL32, and IL32 may play an important role in inflammatory responses and pancreatic cancer growth. Recently, a synergism between IL32 and other well characterized players in innate immunity has been documented.
• Synonyms: IL-32, NK4, TAIF

Reference Data

• Protein Families: Secreted Protein