PML Protein (PML) Mouse Monoclonal Antibody [Clone ID: B3E9]

CAT#: AM09254PU-N

PML Protein (PML) mouse monoclonal antibody, clone B3E9, Aff - Purified

Product Images

Figure.1 Western blot analysis of extracts from K562 cells using anti-PML antibody.

Specifications

Product Data

• Clone Name: B3E9
• Applications: WB
• Recommended Dilution: Western Blot: Use PML antibody at 1/2,000 dilution to differentiate normal PML and Chimeric PML/RAR alpha molecules based on their molecular weight.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant partial Human PML.
• Specificity: This antibody recognizes normal PML proteins of lower Molecular Weight, and also recognizes the High Molecular Weight PML/RAR-alpha Chimeric protein in promyelocytic leukemia patients.
• Formulation: 0.01M PBS, pH 7.4 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore by adding appropriate volume of distilled water to reach a final antibody concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  After reconstitution, aliquot and store at -20°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: promyelocytic leukemia
• Database Link:
  Entrez Gene 5371 Human
  UniProt ID P29590
• Background: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals.
• Synonyms: RING finger protein 71, MYL, TRIM19

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis