Desmoglein 1 (DSG1) Mouse Monoclonal Antibody [Clone ID: Dsg1-P23]

CAT#: AM09135SU-N

Desmoglein 1 (DSG1) mouse monoclonal antibody, clone Dsg1-P23, Supernatant

Specifications

Product Data

• Clone Name: Dsg1-P23
• Applications: IHC, WB
• Recommended Dilution: Immunoblotting (Western).
  Immunohistochemistrry on Frozen Sections: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (See Ref.1)
  Immunohistochemistry on Paraffin Embedded Sections (After Microwave treatment): For better staining on paraffin sections preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media is recommended.
  Recommneded Dilutions: 1/1000 for 1h at RT for Immunohistochemistry (Dilute immediately before use in PBS).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human polypeptide Desmoglein 1 (extracellular part EII, EIII and EIV)
• Specificity: In Immunoblots of cytoskeletal preparations from Human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1.
  In immunolocalisation studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera.
  Cross reaction with Desmoglein 2 and 3 was not detected.
• Formulation: State: Supernatant
  State: Liquid Hybridoma Culture Supernatant (Concentrate)
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: desmoglein 1
• Database Link:
  Entrez Gene 1828 Human
  UniProt ID Q02413
• Background: Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
  Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus.
  Desmoglein 1 is a 165kD glycoprotein that is a core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
• Synonyms: Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DSG1, CDHF4, DG1, DGI

Reference Data

• Protein Families: Druggable Genome