DACT3 Mouse Monoclonal Antibody [Clone ID: 2A5]

CAT#: AM09054PU-N

DACT3 mouse monoclonal antibody, clone 2A5, Purified

Product Images

Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of mouse brain and 293T (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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  Immunohistochemistry: Paraffin embedded sections of human gastric cancer tissue were incubated with anti - human DACT3 ( 1 : 50 ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 2A5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:250 - 500) .
  Immunohistochemistry on Paraffin embedded tissues (1:50).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human DACT3 (aa 89-202) purified from E. coli
• Specificity: The antibody recognizes human and mouse DACT3.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: dishevelled binding antagonist of beta catenin 3
• Database Link:
  Entrez Gene 629378 MouseEntrez Gene 147906 Human
  UniProt ID Q96B18
• Background: DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.
• Synonyms: dapper homolog 3, DAPPER3, RRR1