UNG Mouse Monoclonal Antibody [Clone ID: k1C12]

CAT#: AM09040PU-S

UNG mouse monoclonal antibody, clone k1C12, Purified

Product Images

Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HeLa (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: -N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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  Immunohistochemistry: -N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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  Immunohistochemistry: -N UNG antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thymus at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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Specifications

Product Data

• Clone Name: k1C12
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Immunofluorescence/Immnunocytochemistry.
  Immunohistochemistry on Paraffin Sections (10 µg/ml). Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human UNG (1-313 aa) purified from E. coli
• Specificity: The antibody recognizes Human UNG.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: uracil DNA glycosylase
• Database Link:
  Entrez Gene 7374 Human
  UniProt ID P13051
• Background: The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.
• Synonyms: UDG, DGU, UNG1, UNG15