CD3E Mouse Monoclonal Antibody [Clone ID: PPT3]

CAT#: AM08104PU-N

CD3E mouse monoclonal antibody, clone PPT3, Purified

Specifications

Product Data

• Clone Name: PPT3
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry. (Ref.1)
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections).
  Immunoprecipitation (Ref.1)
  In vitro activation of T cells. (Ref.1)
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes Porcine CD3e (Mr. 23 kDa).
  The Monoclonal antibody PPT3 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q7YRN2
• Background: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains.
• Synonyms: T3/Leu-4