MHC Class II I-Ab Mouse Monoclonal Antibody [Clone ID: 25-9-3]

CAT#: AM08076BT-N

MHC Class II I-Ab mouse monoclonal antibody, clone 25-9-3, Biotin

Specifications

Product Data

• Clone Name: 25-9-3
• Applications: FC
• Recommended Dilution: b>Flow Cytometry: < / = 1 µg/10e6 cells.
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: C3H.SW splenocytes. (Ref.1)
• Specificity: This antibody reacts specifically with the I-Ab haplotype of MHC Class II molecules.
  Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P18468
• Background: MHC Class II antigens are involved in antigen presentation by Antigen Presenting Cells (APCs) to CD4+ T cells. They are constitutively expressed on APCs including B cells, macrophages, monocytes and dendritic cells, and are inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma. The Mouse H2 Ab locus is orthologous to human DQB, which varies from typical class II genes in that both the alpha and beta chains are polymorphic.
• Synonyms: H2-Eb1, H-2 class II histocompatibility antigen I-A beta chain