Cd79b Hamster Monoclonal Antibody [Clone ID: HM79-12]

CAT#: AM08072PU-N

Cd79b hamster monoclonal antibody, clone HM79-12, Purified

Product Images

Immunofluorescent Staining: BALB/c splenocytes were incubated with either Hamster IgG (as an Isotype Control) or purified Hamster anti-Mouse CD79, and then stained with Mouse anti-Hamster IgG (H+L)-FITC. Small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 ug/10e6 cells.

Specifications

Product Data

• Clone Name: HM79-12
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1,2)
  Immunoprecipitation. (Ref.1)
  Immunohistochemistry on Frozen Sections. (Ref.3)
  Western Blotting. (Ref.1)
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Specificity: This antibody recognises beta chain of the mouse B-cell receptor (Mr. 23 kDa).
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD79B antigen
• Database Link:
  Entrez Gene 15985 Mouse
  UniProt ID P15530
• Background: Murine CD79 is a 23/21 kDa disulfide-linked heterodimer composed of an alpha chain (CD79a/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin (Ig) to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage prior to productive Ig gene rearrangements and remaining through all stages of B-cell differentiation prior to plasma cells. It has been proposed that the CD79 complex on pro-B cell surfaces may function to induce early B-cell differentiation. (Ref.1)
• Synonyms: B29, IGB, B-Cell marker