CD103 Hamster Monoclonal Antibody [Clone ID: 2E7]

CAT#: AM08068LE-N

CD103 hamster monoclonal antibody, clone 2E7, Low Endotoxin

Specifications

Product Data

• Clone Name: 2E7
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD103 + cells.
  Immunoprecipitation: This antibody exhibits antigen immunoprecipitation patterns similar if not identical to the Rat anti-Mouse CD103 mAb M290. (Ref.1)
  Immunohistochemistry on Frozen Sections.
  In vitro Activation.
  See Ref.1 for more details.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Specificity: This antibody is specific to CD103/alpha IEL beta7 integrin complex (Mr. 160 and 115 kDa peptides).
  This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation
  is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (Ref.5)
• Formulation: PBS without preservatives.
  State: Low Endotoxin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin alpha E, epithelial-associated
• Database Link:
  Entrez Gene 16407 Mouse
  UniProt ID Q60677
• Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (Ref.1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
• Synonyms: Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE