Ctla4 Hamster Monoclonal Antibody [Clone ID: 9H10]

CAT#: AM08061PU-N

Ctla4 hamster monoclonal antibody, clone 9H10, Purified

Specifications

Product Data

• Clone Name: 9H10
• Applications: FC, FN, IP
• Recommended Dilution: Flow Cytometry: < / = 3 µg/10e6 cells. Suggest use of these reagents is in a final volume 100µl.
  Immunoprecipitation.
  In vitro and in vivo blocking of CTLA-4 action by soluble antibody.
  Promotion of in vitro CTLA-4 signalling by cross-linked antibody.
  See Ref.1 for more details.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Heat-killed Staphylococcus A bacteria coated with mouse CTLA-4/human IgG1 fusion protein. (Ref.1)
• Specificity: This Monoclonal antibody is specific to CD152/CTLA-4 costimulatory receptor (Mr 33-37 kDa).
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cytotoxic T-lymphocyte-associated protein 4
• Database Link:
  Entrez Gene 12477 Mouse
  UniProt ID P09793
• Background: CD152, also known as CTLA-4, is homologous to the costimulatory molecule CD28. (Ref.1,2) Like CD28, the CD152 molecule binds the family of B7 counter-receptors (CD80/B7-1 and CD86/B7-2) on antigen-presenting cells. (Ref.3) CTLA-4 is not expressed on resting T lymphocytes but is induced 2-3 days following T-cell stimulation via the T cell receptor (TCR) complex. (Ref.1,3,4) Whereas CD28 provides a positive costimulatory signal, CD152/CTLA-4 is a negative regulator of T-cell activation, possibly by inhibiting tyrosine kinase signalling through the TCR through its association with tyrosine phosphatases such as SHP-2. (Ref.1,5,6)
• Synonyms: CTLA-4