Cd3e Rat Monoclonal Antibody [Clone ID: C363.29B (YCD3-1)]

CAT#: AM08026PU-N

Cd3e rat monoclonal antibody, clone C363.29B (YCD3-1), Purified

Specifications

Product Data

• Clone Name: C363.29B (YCD3-1)
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry (Ref.1,7-12).
  Immunoprecipitation (Ref.1)
  Functional Assay:
  In vitro Depletion of CD3⁺ cells. (Ref.1)
  In vitro Activation of T cells (accessory cells must be provided). (Ref.1,4)
  Immunohistochemistry on Froszen Sections (Ref.2-4).
  Immunohistochemistry on Paraffin Sections (Ref.5,6).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: IL-4 producing Th2 cell lines including D10
• Specificity: This antibody is specific to CD3e (Mr. 25 kD).
  The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro.
  Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD3 antigen, epsilon polypeptide
• Database Link:
  Entrez Gene 12501 Mouse
  UniProt ID P22646
• Background: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2)
  CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
• Synonyms: T3/Leu-4