Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]

CAT#: AM08024FC-S

Cd5 rat monoclonal antibody, clone 4H8E6, FITC

Specifications

Product Data

• Clone Name: 4H8E6
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD5+ cells (< / = 1 µg/10e6 cells). (Ref.15)
  Immunoprecipitation. (Ref.15)
  Immunohistochemistry on Frozen Sections. (Ref.15)
  T Cell Activation. (Ref.15)
  Western blotting. (Ref.15)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody is specific to CD5/Lyt-1 (Mr. 67 kDa)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD5 antigen
• Database Link:
  Entrez Gene 12507 Mouse
  UniProt ID P13379
• Background: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14)
• Synonyms: CD5, LEU1