LC3B (MAP1LC3B) Mouse Monoclonal Antibody [Clone ID: 5H12]

CAT#: AM06767SU-N

LC3B (MAP1LC3B) mouse monoclonal antibody, clone 5H12, Ascites

Product Images

Western blot analysis using MAP1LC3B mAb against human MAP1LC3B (AA: 1-125) recombinant protein. (Expected MW is 40.2 kDa)

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  Western blot analysis using MAP1LC3B mAb against HEK293 (1) and MAP1LC3B (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).

Specifications

Product Data

• Clone Name: 5H12
• Applications: ELISA, FC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human MAP1LC3B expressed in E. Coli.
  
• Specificity: This antibody reacts to MAP1LC3B.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
  
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 14,6 kDa
• Gene Name: microtubule associated protein 1 light chain 3 beta
• Database Link:
  Entrez Gene 81631 Human
  UniProt ID Q9GZQ8
• Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
• Synonyms: MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3