SKP1 Mouse Monoclonal Antibody [Clone ID: 1H9]

CAT#: AM06720SU-N

SKP1 mouse monoclonal antibody, clone 1H9, Ascites

Product Images

Western blot analysis using SKP1 antibody Cat.-No AM06720SU-N against human SKP1 (AA: 1-160) recombinant protein (Expected MW is 50 kDa).

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  Western blot analysis using SKP1 antibody Cat.-No AM06720SU-N against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and cervical cancer tissues (right) using SKP1 antibody Cat.-No AM06720SU-N with DAB staining.

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  Flow Cytometric analysis of Hela cells using SKP1 antibody Cat.-No AM06720SU-N (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 1H9
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human SKP1 expressed in E. Coli.
• Specificity: Recognizes SKP1 / OCP2
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 19 kDa
• Gene Name: S-phase kinase-associated protein 1
• Database Link:
  Entrez Gene 6500 Human
  UniProt ID P63208
• Background: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
• Synonyms: SKP1A, p19skp1, p19A, OCP-2, OCP-II, EMC19, TCEB1L