SRC Mouse Monoclonal Antibody [Clone ID: 1F11]

CAT#: AM06700SU-N

SRC mouse monoclonal antibody, clone 1F11, Ascites

Product Images

Western blot analysis using SRC mAb against Human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)

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  Western blot analysis using SRC Mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC Mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC Mouse mAb with DAB staining.

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  Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 1F11
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Flow Cytometry: 1/200-1/400.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human SRC expressed in E. Coli.
• Specificity: This antibody recognizes Human SRC. Other species not tested.
• Formulation: State: Ascites
  State: Liquid Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Gene Name: SRC proto-oncogene, non-receptor tyrosine kinase
• Database Link:
  Entrez Gene 6714 Human
  UniProt ID P12931
• Background: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: c-Src, pp60c-src