MSH6 Mouse Monoclonal Antibody [Clone ID: 5B11]

CAT#: AM06693SU-N

MSH6 mouse monoclonal antibody, clone 5B11, Ascites

Product Images

Western blot analysis using CDC2 mouse mAb against Jurkat (1) cell lysate.

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  Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 5B11
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human MSH6 expressed in E. Coli.
  
• Specificity: This antibody reacts to CDC2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 34 kDa
• Gene Name: mutS homolog 6
• Database Link:
  Entrez Gene 2956 Human
  UniProt ID P52701
• Background: This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.
• Synonyms: DNA mismatch repair protein Msh6, GTBP