EIF4E Mouse Monoclonal Antibody [Clone ID: 5D11]

CAT#: AM06602SU-N

EIF4E mouse monoclonal antibody, clone 5D11, Ascites

Product Images

Western blot analysis using EIF4E antibody Cat.-No AM06602SU-N against Hela (Lane 1), HEK293 (Lane 2) and K562 (Lane 3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E antibody Cat.-No AM06602SU-N with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using EIF4E antibody Cat.-No AM06602SU-N with DAB staining.

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  Immunofluorescence analysis of GC-7901 cells using EIF4E antibody Cat.-No AM06602SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometric analysis of Hela cells using EIF4E antibody Cat.-No AM06602SU-N (green) and negative control (purple).

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Specifications

Product Data

• Clone Name: 5D11
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human EIF4E expressed in E. Coli.
• Specificity: Recognizes EIF4E
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 25 kDa
• Gene Name: eukaryotic translation initiation factor 4E
• Database Link:
  Entrez Gene 1977 Human
  UniProt ID P06730
• Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
• Synonyms: eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F